Functional Microscopy

Coordinators: Stavros Taraviras, Professor,  taraviras@med.upatras.gr , Zoi Lygerou, Professor,  lygerou@med.upatras.gr 

Equipments: Leica TCS SP5 Microscope Microscope, equipped with 2 lasers, live cell observation chamber and biomolecule functional imaging software.

Supported Technologies: Confocal Microscopy, Fluorescence Microscopy, 3D-Reconstruction, Fluorescence Recovery After Photobleaching (FRAP), Fluorescence Loss in Photobleaching (FLIP), Förster Resonance Energy Transfer (FRET).

Applications: Co-localization of multiple biomolecules in cells and tissues, functional microscopy, determination of protein interactions and modifications in living cells, analysis of stem cells, functional characterization of medicinal substances.

Microarray Analysis

Coordinator: Konstantinos Stathopoulos, Professor, cstath@med.upatras.gr 

Equipments: ScanArray Express Scanner (Perkin Elmer), HybArray12 Hybridizer (Perkin Elmer), Bioanalyser 2100 (Agilent).

Supported Technologies: Microarray Analysis, Microanalysis.

Applications: Determination of large-scale gene expression, Genotyping, SNP analysis.

RealTime PCR

Coordinator: Adamantias Papachatzopoulou, Associate Professor, apapacha@med.upatras.gr 

Equipments: LightCycler 2.0 (Roche).

Supported Technologies: Quantitative PCR.

Applications: Determination of gene expression, Genotyping, pathogen detection.

Phosphorimager

Coordinator: Dionisis Drainas, Professor, drainas@med.upatras.gr 

Equipments: Fuji FLA3000

Supported Technologies: Imaging, quantification and analysis of samples by fluorescence, chemiluminescence, chemiluminescence, radioisotopes (32P, 35S, 14C, 3H, 125I) and analysis of monochromatic and color samples.

Applications: Detection and quantification of nucleic acids and proteins.

Ultracentrifuge

Coordinator: Dimitrios Kalpaxis, Professor, dimkal@med.upatras.gr 

Instruments: Beckmann’s ultra-centrifuge

Supported Technologies: Centrifuging Biomedical Preparations at High Speed

Applications: Subcellular fractionation, separation and receipt of cells and subcellular organelles, preparation of cell extracts, isolation of viral particles, isolation of nucleic acids and so on.